Publications

Scholarly Journals--Published

  • Hakim MDA, Nye P, Buchholz JN and Behringer EJ Aging alters cerebrovascular endothelial GPCR and K+ channel function: Divergent role of gender. J Gerontol. 75(11): 2064-2073, 2020.   Age-related dementia entails impaired blood flow to and throughout the brain due, in part, to reduced endothelial nitric oxide signaling. However, it is unknown whether sex impacts cerebrovascular Gq-protein-coupled receptors (GPCRs) and K+ channels underlying endothelium-derived hyperpolarization (EDH) during progressive aging. Thus, we simultaneously evaluated intracellular Ca2+ ([Ca2+]i) and membrane potential (Vm) of intact endothelial tubes freshly isolated from posterior cerebral arteries of young (4-6 mo), middle-aged (12-16 mo), and old (24-28 mo) male and female C57BL/6 mice. Purinergic receptor function (vs. muscarinic) was dominant and enhanced for [Ca2+]i increases in old females vs. old males. Although, Ca2+-sensitive K+ channel function as defined by NS309-evoked Vm hyperpolarization, was mildly impaired during female vs. male old age. This sex-based contrast in declined function of GPCRs and K+channels to produce EDH may support a greater ability for physiological endothelial GPCR function to maintain optimal cerebral blood flow in females vs. males during old age. As reflective of the pattern of cerebral blood flow decline in human subjects, KIR channel function decreased with progressive age regardless of sex. Total age-related analyses masked male vs. female aging and, contrary to expectation, hydrogen peroxide played a minimal role. Altogether, we conclude a sex-based divergence in cerebrovascular endothelial GPCR and K+ channel function while highlighting a previously unidentified form of age-related endothelial dysfunction as reduced KIR function.           (05/2021)
  •   Lawrence J., Chen M., Xiao D., Zhang H., Buchholz J.N. and Zhang L.: Fetal Nicotine Exposure Causes PKCe Gene Repression by Promoter Methylation in the Heart. Circ Res. 89: 89-97, 2011   (05/2011)
  •  Charles, SM., Zhang L., Cipolla MJ., Buchholz, JN. And Pearce, WJ.: The roles of cytosolic Ca2+ concentration and myofilament Ca2+ sensitization in Age-dependent cerebrovascular myogenic tone. Am. J. Physiol. Heart and Circ Physiol. 299: H1034-H1044, 2010.   (09/2010)
  •   Goyal, R., Angermann, J.E. Ostrovskaya O., Buchholz, J.N. and Wilson S.M. Enhanced capacitative calcium entry and sarcoplasmic-reticulum calcium storage capacity with aging advanced age in murine mesenteric arterial smooth muscle cells. Exp. Gerontology, 44(3): 201-207, 2009. (05/2009)
  • Buchholz J.N. Behringer E., Pearce, W.J. and Vanterpool, C.K. . "Age-dependent changes in Ca2+ homeostasis in peripheral neurones: Implications for changes in function. ." Aging Cell 6.1 (2007): 285-296. (01/2007)
  • Charles, S.M., Zhang, L., Longo, L.D., Buchholz, J.N. and Pearce, W.J . "Maturation alters calcium dependent sensitivity of pressure dependent cerebrovascular myogenic tone." Am. J. Physiol. Reg. Comp. Physiol 293.1 (2007): R737-R744. (01/2007)
  • Vanterpool, C.K., Vanterpool, E., Pearce, W.J. and Buchholz J.N. "Advancing age selectively alters the expression of the major ryanodine receptor isoforms in adult rat superior cervical ganglia." J. Appl. Physiol 101.2 (2006): 392-400. (07/2006)
  • Zhao, H, Austin, R.R., Christian, S.L., Buchholz, J.N. and Drew, K.L. "Decreased NR1 phosphorylation and decreased NMDAR function in hibernating Arctic ground squirrels ." J. Neurosci. Res 84.1 (2006): 291-298. (07/2006)
  • Xiao, D., Buchholz, J.N., Zhang L. "Pregnancy attenuates uterine artery pressure dependent vascular tone: role of PKC/ERK pathway." Am. J. Physiol. Heart and Circ. Physiol 290.1 (2006): H2337-H2343. (07/2006)
  • Zhao, H, Austin, R.R., Christian, S.L., Buchholz, J.N. and Drew, K.L. . "Decreased NR1 phosphorylation and decreased NMDAR function in hibernating Arctic ground squirrels ." Journal of Neuroscience Research 84. (2006): 291-298. (05/2006)
  • Vanterpool, C.K., Vanterpool, E., Pearce, W.J. and Buchholz J.N. . "Advancing age selectively alters the expression of the major ryanodine receptor isoforms in adult rat superior cervical ganglia." Journal of Applied Physiology 101. (2006): 392-400. (04/2006)
  • Xiao, D., Buchholz, J.N., Zhang L. . "Pregnancy attenuates uterine artery pressure dependent vascular tone: role of PKC/ERK pathway.." Am. J. Physiol. Heart and Circ. Physiol 290. (2006): H2337-H2343. (01/2006)
  • Charles, S.M., Pearce, W.J., Zhang, L., Longo, L.D. and Buchholz, J.N. "Maturation alters calcium dependent sensitivity of pressure dependent cerebrovascular myogenic tone." Journal of Applied Physiology . (): MS#JAP-01271-2005-.

Book Review - Scholarly Journals--Published

  • Hakim MDA, Nye P, Buchholz JN and Behringer EJ Aging alters cerebrovascular endothelial GPCR and K+ channel function: Divergent role of gender. J Gerontol. 75(11): 2064-2073, 2020. (10/2020)

Scholarly Journals--Submitted

  • Hakim MDA, Nye P, Buchholz JN and Behringer EJ Aging alters cerebrovascular endothelial GPCR and K+ channel function: Divergent role of gender. J Gerontol. MS# JGBS-2019-183. Revised 07October, 2019 Hakim MDA, Nye P, Buchholz JN and Behringer EJ Aging alters cerebrovascular endothelial GPCR and K+ channel function: Divergent role of gender. J Gerontol. 75(11): 2064-2073, 2020.   (10/2019)
  • Behringer, E.K., Leite, L.D., Buchholz, N.E., Keeney, M.G., Pearce, W.J., Vanterpool, C.K., Wilson, S.M. and Buchholz, J.N. Development and Long-Term Hypoxia Alters Calcium-Induced Calcium Release in Sheep Cerebrovascular Sympathetic Neurons. Am. J. Physiol. Reg. Comp. Physiol. Submitted. (12/2008)

Books and Chapters

  •  Buchholz JN, Pottorf WJ, Vanterpool CK, Behriner EJ and Duckles SP. Senescence, Chapter title Calcium Regulation in Neuronal Function With Advancing age: Limits of Homeostasis. Publisher, In-Tech Open Access. Accepted September 2, 2011.   (09/2011)
  • Goyal, R., Angermann, J.E. Ostrovskaya O., Buchholz, J.N. and Wilson S.M. Enhanced capacitative calcium entry and sarcoplasmic-reticulum calcium storage capacity with aging advanced age in murine mesenteric arterial smooth muscle cells. Exp. Gerontology, MS# EXG-8561, In Press, Oct. 2008. (12/2008)

Scholarly Journals--Accepted

  • Behringer, E.K., Leite, L.D., Buchholz, N.E., Keeney, M.G., Pearce, W.J., Vanterpool, C.K., Wilson, S.M. and Buchholz, J.N. Development and Long-Term Hypoxia Alters Calcium-Induced Calcium Release in Sheep Cerebrovascular Sympathetic Neurons. J. Appl. Physiol. 107: 1223-1234, 2009.   (07/2009)
  • Behringer E, Vanterpool, C.K., Pearce, W.J., Wilson S.M. and Buchholz J.N. Advancing age alters the contribution of calcium release from smooth endoplasmic reticulum stores in superior cervical ganglion cells. J. Gerontol. Biolog. Sci. , MS #JGBS-08-0076, In Press, Oct 2008. (12/2008)

Abstract

  • (NON-PEER REVIEWED) Erik J. Behringer, Laura D. Leite, Nickolaus E. Buchholz, Michael G. Keeney, William J. Pearce, Conwin K. Vanterpool and John N. Buchholz. Development and Long-Term Hypoxia Alters Calcium-Induced Calcium Release in Sheep Cerebrovascular Sympathetic Neurons. Society for Neuroscience A482.4, 2008. (11/2008)
  • (NON-PEER REVIEWED) Charles SM, Zhang L, Longo LD, Pearce WJ, Buchholz JN. Myogenic tone in juvenile rat MA is resistant to blockade of calcium influx and release of intracellular calcium. FASEB J. A913.3, 2008. (05/2008)
  • (PEER REVIEWED) Charles SM, Zhang L, Longo LD, Pearce WJ and Buchholz JN. . "Generation of myogenic tone requires release of calcium in rat cerebral arteries." FASEB Journal 1.1 (2008): A960.16-. (01/2008)
  • (PEER REVIEWED) Charles SM, Zhang L, Longo LD, Pearce WJ and Buchholz JN. "Generation of myogenic tone requires release of calcium in rat cerebral arteries." FASEB Journal 1.1 (2007): A960.16-. (01/2007)
  • (PEER REVIEWED) Buchholz JN, Vanterpool CK, Pearce WJ, Behringer EK. "Advancing age alters the contribution of release of calcium from internal stores to stimulation-evoked calcium transients." FASEB Journal 1.1 (2007): A945.9-. (01/2007)
  • (PEER REVIEWED) Charles SM, Zhang L, Longo LD, Pearce WJ, Buchholz JN:. "Maturation alters calcium dependent sensitivity of pressure dependent cerebrovascular myogenic tone." FASEB Journal 1.1 (2006): A208.15-. (12/2006)
  • (PEER REVIEWED) Behringer EJ, Vanterpool CK, Pearce WJ and Buchholz JN. "Late maturation and advancing age alter the contribution of release of calcium from internal stores to stimulation-evoked calcium transients." Society for Neuroscience Abstracts 1.1 (2006): A80.5-. (07/2006)
  • (PEER REVIEWED) Charles SM, Zhang L, Longo LD, Pearce WJ, Buchholz JN. "Maturation alters calcium dependent sensitivity of pressure dependent cerebrovascular myogenic tone." FASEB Journal . (2006): A208.15-. (06/2006)
  • (PEER REVIEWED) Charles SM, Zhang L, Longo LD, Pearce WJ, Buchholz JN. "Maturation alters calcium dependent sensitivity of pressure dependent cerebrovascular myogenic tone. ." FASEB Journal . (2006): A208.15 -. (01/2006)
  • (PEER REVIEWED) Charles, S.M., Pearce, W,J., Buchholz, J.N. "Generation of myogenic tone is dependent on at least two pools of calcium in rat cerebral arteries.." FASEB Journal . (2005): A383.3-. (11/2005)
  • (PEER REVIEWED) Vanterpool, C.K., Pearce, W.J., Buchholz, J.N. "Aging Alters Rapid and Spontaneous Intracellular Calcium Store Refilling In Rat Superior Cervical Ganglion Cells." . (2005): A632.8-. (11/2005)
  • (PEER REVIEWED) Vanterpool CK, Pearce, WJ and Buchholz, JN: . "Aging Selectively Alters The Genetic Expression of Ryanodine Channels in Rat Superior Cervical Ganglia." Society for Neuroscience Abstracts . (2005): A214.1-. (08/2005)
  • (PEER REVIEWED) Charles, S.M., Pearce, W,J., Buchholz, J.N. "Generation of myogenic tone is dependent on at least two pools of calcium in rat cerebral arteries. ." FASEB Journal, A383.3 . (2005): -. (01/2005)
  • (PEER REVIEWED) Vanterpool, C.K., Pearce, W.J., Buchholz, J.N.. "Aging Alters Rapid and Spontaneous Intracellular Calcium Store Refilling In Rat Superior Cervical Ganglion Cells. ." FASEB Journal, A632.8 . (2005): -. (01/2005)
  • (PEER REVIEWED) Vanterpool CK, Pearce, WJ and Buchholz, JN. "The Genetic Expression of Ryanodine Channels in Rat Superior Cervical Ganglia." Soc. Neurosci. Abstracts . (2005): -. (01/2005)
  • (PEER REVIEWED) Zhao, H.W., Ross, A.P., Buchholz, J.N., Drew, K.L. "Intracellular [Ca2+] in Cultured Hippocampal Slices of Arctic Ground Squirrels. Soc. ." Society for Neuroscience Abstracts, A963.18 . (2004): -. (11/2004)
  • (PEER REVIEWED) Vanterpool, C.K., Duckles, S.P., Buchholz, J.. "Advancing Age Alters Intracellular Calcium Store Refill In Rat Superior Cervical Ganglion Cells. ." Society for Neuroscienc Abstracts, A735.17 . (2004): -. (11/2004)
  • (PEER REVIEWED) Vanterpool, C.K., Duckles, S.P. and Buchholz, J.:. "Advancing Age Alters Intracellular Calcium Store Refill In Rat Superior Cervical Ganglion Cells. Soc.." Soc. Neurosci Abstacts A735.17 . (2004): -. (01/2004)
  • (PEER REVIEWED) Zhao, H.W., Ross, A.P., Buchholz, J.N., Drew, K.L. "Intracellular [Ca2+] in Cultured Hippocampal Slices of Arctic Ground Squirrels." Soc. Neurosci. Abstracts, A963.18 . (2004): -. (01/2004)